rabbit polyclonal anti sap18  (Proteintech)

Journal: iScience

Article Title: Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells

doi: 10.1016/j.isci.2022.104289

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-SF3B3 (1:1000) , Proteintech , Cat# 14577-1-AP RRID: AB_2270189.

Techniques: Recombinant, Mass Spectrometry, Sequencing, Modification, Protease Inhibitor, Multiplex sample analysis, Multiplex Assay, Fractionation, Cell Culture, Bicinchoninic Acid Protein Assay, Lysis, Cloning, Gene Expression, SYBR Green Assay, Transfection, Ligation, Microarray, TaqMan Assay, Software, Cell Analysis

Journal: Nature Communications

Article Title: DHX9 helicase promotes R-loop formation in cells with impaired RNA splicing

doi: 10.1038/s41467-018-06677-1

Figure Lengend Snippet: DNA–RNA hybrid traps RNA Polymerase II on chromatin. a Depletion of SFPQ and SF3B3 causes retention of RNA Pol II on chromatin that is released by treatment with RNAseH1. Western blot of soluble (cytoplasmic) and insoluble (pellet) fractions of nuclear extracts prepared from HeLa cells that were knocked down with siRNAs against the indicated genes. Blots were also probed for GAPDH and LaminA to validate cytoplasmic and chromatin pellet fractions, respectively. b As in a showing that knockdown of DHX9 in cells depleted of SFPQ reverses the retention of RNA Pol II S2P on chromatin. c RNA Pol II (S2P) is not retained in the insoluble chromatin pellet from cells knocked down for XRN2

Article Snippet: Primary antibodies used in this study: mouse anti-RPA32 (RPA2 Ab #2; Calbiochem) used at a dilution of 1:500 for immunofluorescence experiments (IF) and 1:1000 dilution in western blot (WB); rabbit polyclonal anti-SFPQ (Bethyl Laboratories, A301–320A, IF 1:500, IP 4 μg/ml, and WB 1:1000 dilution), mouse monoclonal anti-SFPQ antibody (Abcam, ab11825, IF 1:500 and IP 3.5 μg/ml dilution), mouse monoclonal anti-GAPDH antibody (GeneTex GT239, WB 1:1000 dilution), rabbit polyclonal anti-RNA polymerase II antibody phospho-ser2 (Abcam, ab5095, IP:2 μg/ml and WB 1:1000 dilution), rabbit Polyclonal anti-RNA polymerase II antibody phospho-ser5 (Abcam, ab52208, IP: 2 μg/ml and WB 1:1000 dilution), rabbit polyclonal anti-Chk1 [p Ser345] (Novus Biologicals, WB 1:1000 dilution), rabbit Polyclonal anti-Phospho RPA32 (S4/S8) Antibody (Bethyl Laboratories, A300-245AWB 1:1000 dilution), rabbit polyclonal anti-RNA polymerase II antibody phospho-ser7 (Abcam, ab126537, IP: 2 μg/ml and WB 1:1000 dilution), rabbit polyclonal anti-SF3B3 antibody (Abcam, ab96683, WB 1:1000 dilution), mouse monoclonal anti-γH2AX (ab26350, Abcam, IF 1:100 dilution and WB 1:500 dilution), rabbit polyclonal anti-53BP1 Antibody (Novus Biologicals, NB100-904 IF 1:500, WB 1:2000, IP 2 mg/ml and NB100-304 IP 2 mg/ml), rabbit polyclonal anti-RNA Helicase A antibody (ab26271, WB 1:1000 dilution and IP 4 mg/ml), rabbit polyclonal anti-XRN2 Antibody (Cambridge Biosciences A301-103A, WB 1:1000 dilution), mouse monoclonal anti-RNase H1 antibody (ab56560, WB 1:1000 dilution) and rabbit polyclonal anti-nucleolin antibody (ab22758, WB 1:1000 dilution), rabbit monoclonal anti-SC35 (SRSF1) antibody (Abcam ab204916, WB 1:2000 dilution).

Techniques: Western Blot, Knockdown